Protein_Domain

Part:BBa_K3629000:Design

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-08-12)


Yarrowia lipolytica XRP2 signal peptide (secretion tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Coding sequence includes the start codon for easy addition to the N-terminus of expression constructs

Source

From genomic sequence of wild-type Y. lipolytica strain CLIB122 CDS: T01033.

References

1. Celińska, E., Borkowska, M., Białas, W., Korpys, P., & Nicaud, J. M. (2018). Robust signal peptides for protein secretion in Yarrowia lipolytica: identification and characterization of novel secretory tags. Applied microbiology and biotechnology, 102(12), 5221–5233. https://doi.org/10.1007/s00253-018-8966-9